Alexandria, VA, USA – A study evaluating the proliferation and osteo differentiation of stem cells from apical papilla (SCAP) stimulated with low-level laser therapy (LLLT) and platelet-rich fibrin (PRF) will be presented at the 101st General Session of the IADR, which will be held in conjunction with the 9th Meeting of the Latin American Region and the 12th World Congress on Preventive Dentistry on June 21-24, 2023, in Bogotá, Colombia.
The Interactive Talk presentation, “A Combination of LLLT and PRF Promotes Proliferation and Osteogenesis,” will take place on Saturday, June 24, 2023, at 3:55 p.m. Colombia Time (UTC-05:00) during the “Photobiomodulation” session.
The study by David Alexander Gutierrez Ramirez of Universidad Antonio Nariño, Bogotá, Colombia, extracted human SCAP from human third molars of donors after obtaining informed consent. The stem cell phenotype was confirmed using specific antibodies (anti-CD34, CD 45, CD105 and CD 90) and flow cytometry analysis. After the stem cell phenotype confirmation, the cells were exposed to PRF and LLLT using LED irradiation (6 J/m2). Cultures were analyzed from the baseline up to 14 days post stimulation. The cell proliferation was evaluated by MTT assay. The bone formation was measured by Alizarin red staining and Image J software. Osteogenic genes including osteopontin (OPN), osteocalcin (OCN) and collagen I (COL I) were analyzed by quantitative real time RT-PCR. The comparisons between groups were performed with Anova analysis and Dunnet Post-hoc test (p<0.05).
The immunophenotype analyses confirmed the extraction of stem cells. The SCAP proliferation was higher in all experimental groups (PRF, LLLT, and PRF+LLLT) in comparison with the control cells (not exposed). The combined exposition to LLLT irradiation and PRF shows higher bone nodule formation in comparison to individual exposition (p < 0.05). Also, SCAP exposed to both therapies showed higher expression of osteogenic genes (p < 0.05).
The stimulation with LLLT and PRF increased both the proliferation and the expression of genes associated with osteo differentiation of SCAPs in comparison to nonexposed cells, suggesting the combination of LLLT and PRF a therapy for better bone regeneration.
About IADR
The International Association for Dental Research (IADR) is a nonprofit organization with a mission to drive dental, oral, and craniofacial research for health and well-being worldwide. IADR represents the individual scientists, clinician-scientists, dental professionals, and students based in academic, government, non-profit and private-sector institutions who share our mission. Learn more at www.iadr.org.